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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/03/2022 |
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Data da última atualização: |
24/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COUTINHO, T. C.; MALAFATTI, J. O. D.; PARIS, E. C.; TARDIOLI, P. W.; FARINAS, C. S. |
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Afiliação: |
ELAINE CRISTINA PARIS, CNPDIA; CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Hydroxyapatite-CoFe2O4 magnetic nanoparticle composites for industrial enzyme immobilization, use, and recovery. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
ACS Applied Nano Materials, v. 3, 2020. |
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Páginas: |
12334–12345 |
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DOI: |
https://dx.doi.org/10.1021/acsanm.0c02811 |
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Idioma: |
Inglês |
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Conteúdo: |
Hydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the βglucosidase biocatalyst, which could be reused 10 times, maintaining MenosHydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes... Mostrar Tudo |
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Palavras-Chave: |
Cobalt ferrite; Immobilization; Magnetic nanoparticles. |
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Categoria do assunto: |
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Marc: |
LEADER 02369naa a2200229 a 4500
001 2141192
005 2024-01-24
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://dx.doi.org/10.1021/acsanm.0c02811$2DOI
100 1 $aCOUTINHO, T. C.
245 $aHydroxyapatite-CoFe2O4 magnetic nanoparticle composites for industrial enzyme immobilization, use, and recovery.$h[electronic resource]
260 $c2022
300 $a12334–12345
520 $aHydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the βglucosidase biocatalyst, which could be reused 10 times, maintaining
653 $aCobalt ferrite
653 $aImmobilization
653 $aMagnetic nanoparticles
700 1 $aMALAFATTI, J. O. D.
700 1 $aPARIS, E. C.
700 1 $aTARDIOLI, P. W.
700 1 $aFARINAS, C. S.
773 $tACS Applied Nano Materials$gv. 3, 2020.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/07/2013 |
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Data da última atualização: |
04/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
SEGALIN, S. R.; HUTH, C.; ROSA, T. D'A.; PAHINS, D. B.; HENNING, L. M. M.; NUNES, U. R.; MARTIN, T. N. |
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Afiliação: |
SAMANTHA RIGO SEGALIN, UFSM; CAROLINE HUTH, UFSM; THAIS D'AVILA ROSA, UFPel; DIÓGENES BARELLA PAHINS, UFSM; LILIANE MARCIA MERTZ HENNING, CNPSO; UBIRAJARA RUSSI NUNES, UFSM; THOMAS NEWTON MARTIN, UFSM. |
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Título: |
Foliar application of silicon and the effect on wheat seed yield and quality. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Seed Science, Londrina, v. 35, n. 1, p. 86-91, 2013. |
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ISSN: |
2317-1537 |
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DOI: |
10.1590/S2317-15372013000100012 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: Adequate nutrients for plants are important for increasing the yield and quality of the seeds produced. The objective of this study was to evaluate foliar fertilization with silicon in wheat and its effect on seed yield and physiological quality. Treatments consisted of two silicon dosages (three and six liters silicon per hectare) and the control (no silicon) and five wheat cultivars: OR "Quartzo", OR "Ônix", Fundacep "Linhagem", Fundacep "Campo Real" and Fundacep "Horizonte". The experimental design was randomized blocks with four replications. After physiological maturity, harvesting and threshing of the seeds were done manually. Seed samples were evaluated for yield and physiological quality from the germination test, first germination count, seedling dry weight, accelerated aging, electrical conductivity, 1000 seeds and hectoliter weights. The results showed that the foliar application of silicon at the dosages tested did not affect the yield and physiological quality of the seeds produced by the wheat cultivars. RESUMO: A disponibilidade adequada de nutrientes às plantas é de fundamental importância tanto para incrementos em rendimento bem como, para a produção de sementes de elevada qualidade. Dessa forma, objetivou-se com esse trabalho avaliar o efeito da adubação foliar com silício em trigo, no rendimento e na qualidade fisiológica das sementes produzidas. Os tratamentos consistiram de duas doses de silício aplicados via foliar (três e seis litros de silício por hectare), além da testemunha (sem aplicação do produto). Foram utilizadas cinco cultivares de trigo (OR "Quartzo", OR "Ônix", Fundacep "Linhagem", Fundacep "Campo Real" e Fundacep "Horizonte"). O delineamento experimental foi blocos ao acaso, com quatro repetições. Após a maturidade fisiológica, efetuou-se a colheita e a debulha das sementes de forma manual. Posteriormente, as amostras foram encaminhadas ao laboratório de análise de sementes onde foram efetuadas as avaliações do rendimento e da qualidade das sementes, por meio dos testes de germinação, primeira contagem da germinação, massa seca de plântulas, envelhecimento acelerado, condutividade elétrica, massa de mil sementes e massa hectolitro. A aplicação de silício, nas cultivares de trigo e nas doses testadas, não afeta o rendimento nem a qualidade fisiológica das sementes produzidas. MenosABSTRACT: Adequate nutrients for plants are important for increasing the yield and quality of the seeds produced. The objective of this study was to evaluate foliar fertilization with silicon in wheat and its effect on seed yield and physiological quality. Treatments consisted of two silicon dosages (three and six liters silicon per hectare) and the control (no silicon) and five wheat cultivars: OR "Quartzo", OR "Ônix", Fundacep "Linhagem", Fundacep "Campo Real" and Fundacep "Horizonte". The experimental design was randomized blocks with four replications. After physiological maturity, harvesting and threshing of the seeds were done manually. Seed samples were evaluated for yield and physiological quality from the germination test, first germination count, seedling dry weight, accelerated aging, electrical conductivity, 1000 seeds and hectoliter weights. The results showed that the foliar application of silicon at the dosages tested did not affect the yield and physiological quality of the seeds produced by the wheat cultivars. RESUMO: A disponibilidade adequada de nutrientes às plantas é de fundamental importância tanto para incrementos em rendimento bem como, para a produção de sementes de elevada qualidade. Dessa forma, objetivou-se com esse trabalho avaliar o efeito da adubação foliar com silício em trigo, no rendimento e na qualidade fisiológica das sementes produzidas. Os tratamentos consistiram de duas doses de silício aplicados via foliar (três e seis litros de silíc... Mostrar Tudo |
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Thesagro: |
Trigo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94942/1/Foliar-application-of-silicon-and-the-effect-on-wheat-seed-yield-and-quality.pdf
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Marc: |
LEADER 03063naa a2200229 a 4500
001 1961879
005 2022-04-04
008 2013 bl uuuu u00u1 u #d
022 $a2317-1537
024 7 $a10.1590/S2317-15372013000100012$2DOI
100 1 $aSEGALIN, S. R.
245 $aFoliar application of silicon and the effect on wheat seed yield and quality.$h[electronic resource]
260 $c2013
520 $aABSTRACT: Adequate nutrients for plants are important for increasing the yield and quality of the seeds produced. The objective of this study was to evaluate foliar fertilization with silicon in wheat and its effect on seed yield and physiological quality. Treatments consisted of two silicon dosages (three and six liters silicon per hectare) and the control (no silicon) and five wheat cultivars: OR "Quartzo", OR "Ônix", Fundacep "Linhagem", Fundacep "Campo Real" and Fundacep "Horizonte". The experimental design was randomized blocks with four replications. After physiological maturity, harvesting and threshing of the seeds were done manually. Seed samples were evaluated for yield and physiological quality from the germination test, first germination count, seedling dry weight, accelerated aging, electrical conductivity, 1000 seeds and hectoliter weights. The results showed that the foliar application of silicon at the dosages tested did not affect the yield and physiological quality of the seeds produced by the wheat cultivars. RESUMO: A disponibilidade adequada de nutrientes às plantas é de fundamental importância tanto para incrementos em rendimento bem como, para a produção de sementes de elevada qualidade. Dessa forma, objetivou-se com esse trabalho avaliar o efeito da adubação foliar com silício em trigo, no rendimento e na qualidade fisiológica das sementes produzidas. Os tratamentos consistiram de duas doses de silício aplicados via foliar (três e seis litros de silício por hectare), além da testemunha (sem aplicação do produto). Foram utilizadas cinco cultivares de trigo (OR "Quartzo", OR "Ônix", Fundacep "Linhagem", Fundacep "Campo Real" e Fundacep "Horizonte"). O delineamento experimental foi blocos ao acaso, com quatro repetições. Após a maturidade fisiológica, efetuou-se a colheita e a debulha das sementes de forma manual. Posteriormente, as amostras foram encaminhadas ao laboratório de análise de sementes onde foram efetuadas as avaliações do rendimento e da qualidade das sementes, por meio dos testes de germinação, primeira contagem da germinação, massa seca de plântulas, envelhecimento acelerado, condutividade elétrica, massa de mil sementes e massa hectolitro. A aplicação de silício, nas cultivares de trigo e nas doses testadas, não afeta o rendimento nem a qualidade fisiológica das sementes produzidas.
650 $aTrigo
700 1 $aHUTH, C.
700 1 $aROSA, T. D'A.
700 1 $aPAHINS, D. B.
700 1 $aHENNING, L. M. M.
700 1 $aNUNES, U. R.
700 1 $aMARTIN, T. N.
773 $tJournal of Seed Science, Londrina$gv. 35, n. 1, p. 86-91, 2013.
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